![]() ![]() The most well-known example of a DNA-binding dye is SYBR Green. The amount of fluorescence emitted upon DNA-binding of the dyes is directly proportional to the amount of dsDNA present. The chemistry of these types of dyes might be simple, but they are extremely effective. Real-time detection of PCR products can be achieved through the use of either: 1) DNA-binding dyes or 2) fluorescent PCR primer- and probe-based chemistries.Īs their name implies, DNA-binding dyes are non-specific fluorescent dyes that intercalate with any double-stranded DNA (dsDNA) molecules. Let’s begin our discussion of probes, shall we? A Little Intro…Īs previously mentioned, the goal of qPCR is to monitor the amplification of a target DNA molecule (either DNA or cDNA) during PCR rather than at the end of the reaction. Whew! I don’t know about you, but I sure feel better having gotten all of that off of my chest. Following reverse transcription of mRNA into cDNA, cDNA is amplified by qPCR and the data can be used to (1) quantify the concentration of the RNA target present in the sample (by using a standard curve), or (2) determine relative quantification for gene expression analysis. RT-qPCR, or reverse-transcriptase quantitative PCR, is, as you probably guessed, a combination of the two methods mentioned above.TIME.) called “real time PCR” and shortened to “RT-PCR”. ![]() Thus, it is often (and by often I mean ALL. qPCR refers to quantitative PCR, which allows for the quantification (both full and semi-) of target DNA in real time, as opposed to conventional or end-point PCR, where DNA is quantified at the end of the PCR run.RT-PCR is short for reverse-transcriptase PCR, and is used to detect RNA expression via the use of reverse transcriptase to generate complementary DNA (cDNA).Sooo confusing, amirite? They all refer to specific molecular biology assays, but the names are unfortunately used interchangeably, which can be awfully confusing for just about anyone. Before we go any further, we have to get some things straightened out: RT-PCR versus qPCR versus RT-qPCR. ![]()
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![]() ![]() 6-channel control room monitoring system supports mono through 5.Full transport and navigation controls, including location commands and scrub/shuttle capability.The internal Talkback mic requires an external phantom. A DB25/XLRM that goes from the X-Mon main outputs and connects to the XLR cables that feed the 5. Custom Fader Mode for flexible channel and parameter mapping XMON provides 18 V phantom power for the external Talkback Mic that connects via the DB-25 connector.Flip Mode for transfer of parameters from rotary controls to faders To me, it seems that not being able to monitor your cue mix within the control room when setting it up the cue mix is a MAJOR oversight in the design of the D-Command/XMon.Dedicated controls for automation mode, enable and safe status.(not included), and attach the control lead from the XMON to the D-Control or D-Command Main Unit. On the back of XMON are four D-Sub connectors for inputs. Dedicated controls for all channel strip functions including recording and input monitoring modes, mute, solo, and channel select Digidesigns D-Command and D-Control represent perhaps the most ambitious attempt yet to integrate recording and mixing systems.Dedicated EQ and dynamics plug-in control sections.Flexible display of pan, insert, send, plug-in and mic pre controls.Dedicated controls for assignment and activation of inputs, outputs, inserts and sends.Touch-sensitive, multi-purpose rotary controls.Touch-sensitive, motorized 100mm faders.It´s not complicated, just needs a bit of attention. Digidesign XMON Monitor System 450 61 price drop 389 Used Very Good Add to Cart Digidesign Avid XMON Interface 399.99 20 price drop 379.99 Used Very Good Add to Cart Digidesign Avid XMON Interface 389. ![]() I enclose a link to the pinouts and also to a diagram showing where stuff needs to be plugged. If you have both an Omni and an AVID I/O, choose one of the converters to output the regular PT buss (stereo or 5.1) and connect it on the Xmon respective Dsubs connectors. hi all, question regarding anyone who knows about the ICON setup for speakers. You need a breakout cable with Dsub to female xlr´s to connect the male xlr´s of the BM outputs. 1 knifetheglitter Registered User 15 years Digidesign ICON D-Command speaker output help. If you use the BM card outs 12&13 to output the MAC audio, you need to connect it to one of the 3 Stereo inputs available on the Dsub 25 pin on the rear of the Xmon. The cable must follow the exact pinouts as described on your D Command guide, otherwise it will be messed up. What type of breakout cable are you using to connect your MAC outs (via BM outs) to the Xmon stereo inputs? It seems that the problem lies there. ![]() ![]() We had this working with Pro Tools HD 12.4 for whatever that is worth. We had an avid tech install a new LED power supply as well as a new fader supply early this year. You connect the gear and that´s it, all operations take place on the surface. 1 04-17-2017, 05:50 PM sjtae Member Join Date: May 2012 Location: South Korea Posts: 12 96I/O, XMON, D-command connection Hi guys, I am using PT HD9, connected with 96 I/O, XMON and D-command. Avid / Digidesign D-Command with Xmon Monitor Rack. There´s no app or control panel for Xmon. Is there any possibility to make this problem because of it? I have same audio out for left or right even centre.Īlso I have HD native card and omni in my system as well. Is there any software to control Xmon like avid coreaudio manager?Īnd also on sound option in system preferences of my mac, balance for control BM pan does't work any more. It works but for all of them its mono and I have to push Stereo 2 or 3 on icon. Each D-Command unit communicates with Pro Tools using. I check BM 12 and 13 audio out by connected each other to for example ST 3 or 4 or 5 on xmon Lastly, check if you don´t have the Mono button pressed on the D Command by accident It should work fine, where I work we have the BM Decklink Studio outs 12&13 connected to the D Command stereo in1 as you´re doing. No donations for this lot Auction begins to close at 7 PM on Monday, August 23, 2021. Not sure what audio you are using the BM outs for, but whether it´s the desktop video app audio outs or your computer audio outs, check both settings on the respective interfaces. Provides controls for most Pro Tools recording, editing and mixing tasks, and a versatile remote-controlled audio monitoring system. Are you connecting the BM outs to the right pair of inputs on the DB25 stereo ins on the Xmon?Eg. If both channels output, then the problem is at the breakout cable that connects to the XMon. If you only have the right channel, switch it on the BM outs to check if it´s the breakout cable on the BM. ![]() ![]() ![]() It lets designers transform their boring stuff into 3D stuff. ![]() MeshMixer is a designing application for giving your virtual idea with solid shape. The list of technical features includes easy-to-use, ZBrush navigation controls, free moving navigation, hotkeys toggled on/off the system, various plugins for advanced projects, immersive interface, optimizing brushes, etc. Sculpting, dynamic tessellation, UV texture painting, and cavity painting are its primary functions. The other best thing about Sculptris is its user-friendly relation external environment, and that is the import of 3D meshed (.obj) into the Sculptris for further detailing generation normal and displacement maps. Sculptris lets designers and artists pull, pinch, push, and twist the virtual clay to make what they want. Sculptris is developed on the concept of modeling clay, and its focus is entirely on the same idea. The two main functions are the availability of 3D sculpting and 3D painting in addition to others. It comes for entering into the world of digital sculpting and involving yourself to create the best products. Sculptris is a virtual sculpting tool designed for beginners to experts. Permanent interactive sliders, a user-friendly interface, powerful stylus enhancement, multi-monitor, and font manager are key features of the solution. The graphic design solution introduces the high-caliber LiveSketch tool, and this tool features an open-enabled device that instantly allows you to capture your original idea. CorelDraw allows you to create your ideal workspace with your favorite devices, icon size, color, and all the other related things. It provides you with the freedom to customize your design space. It also offers a reliable workspace designed to match your workforce needs and helps you be productive. ![]() The solution has lots of different tools that make it simple to get started and also provide versatile in-product learning high-quality content that allows you to unleash your creativity right from the start. It is simple and easy to use software specially designed for experienced designers and first-time users. It comes with a suite of many cutting-edge features that helps users to create beautiful photos, design, graphics, and websites. CorelDraw is the world’s most leading Graphic Design Software solution created by Corel Corporations. ![]() ![]() ![]() Not only good clothes in a beautiful location, but the #3Dcreator is great at receiving feedback and suggestions and always open for a chat which is a total plus in our mystery shopper’s book! □ So don’t hesitate to sign the guestbook and leave your comments, rate this 3D location because your opinion matters! The style tends more towards #darkfashion in virtual but even if that is not exactly your own personal style, you will still want to visit this shop in Twinity, as you are certain to find something to suit your #VWclothingtaste as well. It is a large #3Dlocation with separate rooms for both Male and Female clothing. In case you missed it, check out Mystery Shopping in virtual worlds here!įirst stop in our mystery shopping tourtis GMV Queen! If you’re a Twinizen, then this won’t need much introduction! And while Vampire Queen, the owner of this #Online3DShop has another location, to hopefully open soon, she has done a great job here. #DressUp as your most beautiful and join this happy occasion! More info here and updates before and after the wedding will be posted on Twinity’s Facebook and Twitter.ģD Twinity dating site adult site find love virtually marriage online online true love online wedding Virtual St. Without further ado we extend Tricia and Geno’s invitation to virtual St. Paul’s Cathedral, that inspired the post. Making planning less difficult, bringing friends and family from all over the world together to witness the happy event.Īctually it was Tricia’s and King Geno’s wedding and request to have their event performed in St. No matter how far you are from each other a # virtualwedding ceremony can bring a wedding date closer. If you can fall in love in a virtual world and have a relationship that can last for years, marriage is the next step, be it in virtual or if you meet in real and get married, as quite a few Twinizens already have. Take advantage of another “add to cart kind of day” in Twinity now! □ Join us for free here and you’ll see exactly what I mean!ģD store adult site Couples Poses couplesgoals free avatar free dating site Free virtual games online shopping Online Virtual Games RP teenage virtual world games Twinity life dating site virtual chatting games virtual life virtual relationship virtual shopping virtual world game virtual worlds virtuallove VRĪfter our other posts about love in #VirtualWorlds , we thought it’s about time to talk a bit about getting married in a virtual world. Remember to sign the guestbook and rate the places your visit! Be a mystery shopper yourself and let us know what you loved or what you’d change in this place? If you’re a Twinizen you know that we just love to mix shopping with parties so make sure you watch out for #onlineevents held here. If you’re a creator and you have a #3DShop you can contact support and submit your shop for the Globe and Business Directory.Ĭalle’s #3DShop called Pose recently added to Globe menu has a variety of couples and individual poses for you to choose from and enjoy with your special someone or with your #onlinefriends.įrom poses you can place anywhere when you decorate your own home in virtual to furniture items that include pre-set poses for general audience. Or rather shop this pose! #MysteryShopping at the new Go Shopping Globe addition. ![]() ![]() With this in mind, we’re thankful for bringing this issue to our attention, as we’re always looking for ways to better our service, and your feedback is an invaluable part of that process. We do our best to train our agents on how to properly handle each case, including how to escalate problems that they’re unable to assist with, sadly your concern was not handled properly, and we are going to take the necessary steps to ensure that this situation does not repeat itself. We always pride ourselves on giving 100% every day to ensure that each of our customer’s needs are being met, and we know that we have let you down, and for that, we’re very sorry. Make sure to list them including the skills you gained.Our sincerest apologies, Kristen, for the negative experience, that you had with our agent, we realized that it was unmannerly in solving your query, and we completely understand your frustration. If you have done these activities during your study, they are refered to as an extracurricular activity. For instance by doing voluntary work as a coach, trainer or accountant for a club or organization. You can also gain certain skills and competencies in a non-professional setting. Make sure to mention whether you earned a diploma or certificate! ![]() If you have followed any and they’ve improved skills or competencies that are relevant for your new job, make sure to include them. Some employers offer courses or trainings to improve certain skills of their employees. These strong personal traits are gained through experience and will help you execute any other job more efficiently. ![]() However, during your career, you gain competencies and skills which are transferable. In this paragraph, which is read by most recruiters, you will get the chance to sell yourself in a few sentences the kind of role you are looking for, your qualities and ambitions.Īll jobs are different of course. Most modern resumes include a short introductory paragraph called personal statement or profile. Therefore, consider adding the following sections to your resume You want potential employers to see that you are the best fit for the job. However, you if you really want to stand out from other applicants, it is strongly advisable to put in a little more effort. The aforementioned parts should be present on any resume, at any application. Also name the school or institution where you studied, the starting date and date of graduation. Just like the previous overview of work experience, start of with your last study or highest level of education. Tip: try and list skills and responsibilities most relevant to the role you’re applying for! Per job, give a short clear summary of your tasks, responsibilities and skills. Start with your latest job and continue with the jobs you worked at before. ![]() In an a-chronological order, list your latest work experience. In case you have a representative LinkedIn profile or personal website, you can include a link to it in the personal details section. In case a driver's license is required for the role, also mention it. Also mention your place of residence and address, as an employer might prefer an employee living nearby. Therefore, you always start by mentioning your full name and email address and (mobile) phone number. Of course, your new employers should be able to contact you for a job interview. However, the bare minimum of details on your resume should at least include So that means, what should be in your resume can differ per application. Your resume should only contain information relevant to your potential employer. ![]() ![]() ![]() TV-out you can find one for about the same amount as a basic card. If you've got a video card with TV-out support then you should be able to connect your PC directly to your television and view DivX and XviD videos on it. You can find helpful information about video conversion/ authoring Player doesn't support playback of DivX® or XviD you first have to convert the video to MPEG-2 and then record it as a DVD or SVCD, depending on what formats your player actually If your DVD-player supports DivX® and/or XviD playback then you simply need to burn the movie to a CD/DVD and it should play right away. How do I play downloads in my DVD-player?įirst of all, we suggest that you check the DVD Player Compatibility list to figure out if yourĭVD-player supports DivX® and/or XviD playback or not. Server you might be able to achieve similar times to these, but usually your movie downloads will most likely take a bit longer. When you download from a fast and reliable Please note that the transfer times displayed above are calculated based on the maximum download speed of your Internet connection. Table below should give you an idea of how long it would take to download a 700MB file (typical movie download) using your Internet connection if we assume there are no limitations on It will always help to have a fast Internet connection, but your download speed will also be limited by the Internet connection of the source you're downloading the movie from. How long does it take to download a movie? If a movie is longer than 2 hours it might need even more space to ensure that the quality is acceptable. You should expect a typical DivX or XviD movie to beĪround 600MB-700MB in size. The file size of movie downloads vary a lot based on the runtime of the movie and the bitrate used when encoding it. Video codec such as DivX® or XviD and that they originate from a good source. To get the best possible quality when you download movies, make sure that the movies use a If the movie originates from a good source then there are pretty much twoįactors that affect the quality of the movie, the size and the video codec used. One of the main factors that determine the image quality of movie downloads is where the movie came from. To resolve common playback problems with downloads, please check out our DivX FAQ and XviD FAQ. What can I do if I'm having problems playing downloads? That you need to install the AC3 codec in order to get surround sound. To play XviD videos you need to install the XviD codec. ![]() In order to play DivX videos youįirst need to install the DivX codec. The only thing that's needed to view downloads is a media player of your choice and the necessary video and audio codecs installed on your system. The availability of free movies has also received quite a lot of coverage in the press lately. People to download movies in shorter times than before. Also, the growing availability of fast Internet connections in homes have enabled The most important factor is the creation of more powerful video and audioĬompression formats that allow movies to be compressed and transferred much faster than previously. There are numerous factors that have helped to make downloadable movies popular the last few years. What has made movie downloading so popular? The only thing that's required to get started downloading movies is a computer and an Internet connection, then you're all set. Just start a new download and within a couple of hours you will have a movie download ready to view without leaving Most likely because it's a fast and easy way to get movies. You can also find some web sites such as the Moving Image Archive that provide free movie downloads. It is created for the sole purpose of making it easy to download files. While there are numerous ways to download free videos online the quickest and easiest way to download videos is by using file-sharing technology. Download DivX Movies Where can I download free DivX movies? ![]() ![]() ![]() The value of your ex partner’s pension could be offset against your own retirement savings, or other assets such as the family home.Ĭan we draw up the settlement by ourselves?Ĭouples living in England and Wales can sort out their financial settlement themselves.Part of your ex’s pension pot now, which is transferred into your own pension scheme.Will you continue to pay the premiums? Will the beneficiaries for life insurance change? 3. Or if you have a life insurance policy, you will need to agree how each policy will be dealt with. Try and build up a picture of your current state of affairs and work out:įor example, if you have mortgage together, can one of you afford to keep up with repayments? You should also assess your financial situation, both as individuals and a couple. Check out our article on whether a joint account is a good idea. ![]() If you think your ex may go on a spending spree you can seek to freeze your cards. Make sure any wages or benefits go into a new separate account. If you have a joint bank accounts, loans or credit cards, contact your bank or building society as soon as you know you are separating. You need to agree with your ex who gets what and how you will continue to pay your bills. A financial settlement divides savings and property and also covers child maintenance How do I prepare for a financial settlement?īefore you look to reach an agreement, you should sort out your day-to-day finances. This can also be used where the parent liable for payment is particularly wealthy and if there are education expenses to be covered. If a parent who is supposed to pay maintenance lives abroad, you can make an application to the court for a child maintenance order. It will work out how much should be paid, taking into account who the children live with and how much each parent earns. You can either make an arrangement of who pays what between the two of you, or get the Child Maintenance Service involved. If there are children involved, both parents are expected to pay towards the cost of raising them until they are at least 16. To reach a settlement you also need to consider the division of any debt, loans or credit cards you both have. These are usually protected by a pre-nuptial agreement, if one is in place. The financial assets that were acquired before or after the marriage are considered a non-matrimonial asset. In other words, those that were acquired during the marriage. Non-matrimonial assets are treated differently to matrimonial assets. Financial support such as Child maintenance and Spousal maintenance payments.Find out more about property rights and divorce here. Property, including the family home and any property they own individually.Money, including savings and investments.What you are entitled to in a divorce depends on a number of factors and there are no specific guaranteed entitlements for either party.Įach situation is unique and will be treated as such by the courts, but the type of things you might be entitled to include matrimonial assets such as: ![]() What am I entitled to in a divorce settlement? Read more here on how to cut the cost of divorce. The law is different in Scotland because you can’t make a claim for financial provision after divorce.ĭivorce law changed in April 2022 but this will not affect the outcome of financial settlements. NOTE: In England and Wales, getting divorced does not end your ability to make any financial claims against the future earnings your ex, or them against you. It needs to be approved by a judge, after the first part of the divorce process (decree nisi) has been pronounced. This legal document confirms both parties’ agree on the division of assets. Once you have reached an agreement, a solicitor will draft a consent order to make it legal binding. ![]() You can draw one up at any point during divorce proceedings or civil partnership dissolution. A financial divorce settlement is an agreement between you and your ex on how to separate your money and assets once the marriage is over. ![]() ![]() ![]() 1985 – On March 9, the game begins to have drawings every day of the week.1984 – Two years after its debut, Pick 4 begins to have six drawings per week, starting on August 27. ![]() 1982 – Debut of the Pick 4 game with drawings on Tuesdays and Fridays only.Illinois Pick 4 has faced some changes in its drawings and features since its creation almost 40 years ago: He got his prize on September 11, 2020, and the first thing he did was to tell his wife about the prize. Her friend from work was the first person to know about her prize.Īnother featured Pick 4 winner was Gilroy, also from Chicago, who took a $1,040 prize that was not his first one after 30 years of playing the lottery. We got to know Phyllis, a Chicago woman who won $13,000 on September 25, 2020, after 20 years of playing the local games. The Illinois Lottery focused on highlighting random Pick 4 winners. If you make that option, consider taking a minute every day to check the Illinois Pick 4 results on this page. In total, that represents 12 drawings counting with both midday and night drawings. Through the Advance Play feature, players can spare some time by purchasing tickets for up to 6 consecutive days at once. Double the ticket price to add Fireball.Bookmark this page and save your receipt from claiming your prize after you check the Illinois Pick 4 results here.Finally, ask yourself if it is worth adding the Fireball feature for an extra cost and finish by paying for your games.Decide for the type of bet you want to place according to our Prizes & Odds table and consider the odds and prizes.Your physical or digital play slip must be completed by choosing four numbers between 0 and 9 each for each panel that you want to play.If you are not in that group, there are several other lottery games available on the best-ranked lottery sites that we reviewed. Illinois Pick 4 can be played online or at local retailers, but it is only available for people from the state.The Fireball add-on works just like in the Florida Pick 4, doubling the cost and improving the odds.Winners have up to a whole year to collect their Illinois Pick 4 prizes.Two daily drawings occur throughout the week.The maximum possible prize is $25,000 for the ticket with the highest cost for a Straight bet.Ticket cost starts at $0.50, but it also can cost $1, $2, $3, $4, or $5, and the prizes adjust accordingly.The two smallest ticket prices accept Combo plays that include combinations as Straight bets.Tickets worth at least $1 can bet on both Straight and Box plays at the same time.Straight bets require that you match all the numbers and their order, while Box bets include several combinations from 4 to 24 different ways. ![]() ![]() ![]() Selecting elements on the page and moving them where you want is very seamless. What I like is that the editor is very intuitive to use. While not as flexible as Instapage’s landing page editor, it is still very nice and it can be used to achieve just about any design you desire. After that you can use your mouse to drag elements around and put them where you want them. Pages in Dotcomsecrets are laid out using sections that can have any number of columns and rows. While Expert Secrets Russell Brunson Free Pdf gives you a wonderful starting point with its funnel and page templates, the Expert Secrets Russell Brunson Free Pdf editor is the place you can truly make each page in your funnel your own. Your sales funnel is now live and you can start sending traffic to it. Expert Secrets Russell Brunson Free Pdfīest Feature #2: Good Visual Drag and Drop Editor Add your products and ensure all your email and payment integrations are set up.Customize each page to fit your business.Here’s a high-level overview of how to create your first sales funnel: So even if you don’t have any design skills, your funnel pages will have a consistent, professional design. Moreover, each funnel type has its own group of free templates and paid templates. Or to more complex sales funnels like the 2-Step TripWire Funnel which sells a very low ticket front-end product (your tripwire) and then upsells them to a more expensive products with Order Form bumps, and One Click upsells or Downsells. They include simple funnels like the Squeeze Page Funnel which collects a visitor’s email address and then sends them to a Thank you page when signing up. But there are some other special funnels like Membership funnels you can create too. The funnel forms fall into 3 main classes: Lead Capture Funnels, Sales Page Funnels, and Event Funnels. Russell has identified 22 different forms of funnels that he has used successfully in his own business. ![]() In case you’ve never created a sales funnel before then Expert Secrets Russell Brunson Free Pdf has you covered. That is amazing.Expert Secrets Russell Brunson Free Pdf What are the best parts of Dotcomsecrets?īest Feature #1: Funnel template blueprints If you check out their 2 Comma Club site, you can see that almost 300 entrepreneurs have already created 1 million dollar funnels using Dotcomsecrets. – Every type of sales funnel you can think of, complete with order bumps, upsells and downsellsĪdditionally, with the highest end Etison Suite package you will even have the ability to start your own affiliate program (Backpack) and change out your email service with (Actionetics MD).īasically, you’re getting everything you need to market and sell your products online, without having to employ expensive developers.Īnd individuals are utilizing Dotcomsecrets with great success. With a bunch of added bells and whistles. In its core, Dotcomsecrets is an application for constructing sales funnels. Since that time, Russell has turned Dotcomsecrets into a $100 million per year company and they just crossed over 65,000 active members as of August 2018.
![]() ![]() Her cries upon her death in hell reverberated all the way to the surface world. It is said that all were smitten by her beautiful and talented body and mind. The 21st leader of the Getsu clan is known as the Getsu clan's greatest female ninja. Fearsome bosses and ever-changing layouts will challenge players while allowing them to grow stronger with each attempt. The game features highly strategic combat based on the spacing and timing aspects unique to Japanese martial arts. The game will also feature a soul system. Weapons will be customizable via a crafting system. The player takes the role of Getsu Fuma and will acquire many moves throughout the course of the game inspired by Japanese martial arts, as well as other supernatural moves. The game is a hack-and-slash platformer game in a side-scrolling view featuring a Japanese-styled theme in a ukiyo-e art style. Īs the chosen leader and guardian of the land of the living, wield the otherworldly arsenal and powers of the Getsu Clan as you descend deeper into the depths of Hell to eradicate the source of the cataclysm. As it stands at the brink of destruction, Getsu Fuma must take on the helm as the leader of his clan and delve into the pits of Hell to rip out the evil at its core. – Prologue from the official site Plot Īfter 1,000 years of peace, the seal to the gate of hell has been ripped open and a flood of evil spirits have been released into the world. Now, their heroic 27th leader must descend into that vile abyss to root out the evil behind this cataclysm. The Getsu clan has kept watch over the underworld ever since the first Fuma defeated Ryukotsuki. Synopsis Getsu Fuma: Descent to the Underworld Ryukotsuki has returned after a thousand years, undoing the seal upon hell and bringing forth monsters and evil spirits who threaten the end of the world. ![]() |
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